Exhaustive analysis using DGGE method for oral flora causing the infective endocarditis

我们建立了基于Rod形状决定蛋白基因(RodA)变异的DGGE方法，以口腔中引起感染性心内膜炎(IE)的最大细菌--口腔链球菌为靶标。在此基础上，对唾液标本中的细菌菌群进行了详尽的分析。此外，我们通过生化特性检查和来自一名感染IE患者的菌株的16S rRNA基因来估计细菌菌株。我们测定了经变形链球菌鉴定的菌株的基因组DNA草稿序列，并与数据库中已公布的基因组序列进行了比较。然后，我们回顾了IE特有的发病因素。采用BML公司的龋病检查、牙周病检查、围栏细菌检查等方法，对与龋病有关的细菌种类、牙周致病菌进行了研究，对机会感染进行了调查。

We established DGGE method based on the variety of the Rod shape-determining protein gene(rodA) as a target the oral streptococcus which was the maximal bacterial species in the oral cavity causing infective endocarditis(IE). Based on this, we performed exhaustive analysis of the bacterial flora as saliva specimen. Also, we estimated bacterial strain from an examination for biochemical property and a 16S rRNA gene of the strain from a patient who has contracted IE. We determined a draft sequence of the genomic DNA of strain identified with Streptococcus mutans and performed comparison between genomic sequence that was already presented on a database. then, we reviewed the pathogenesis factor that was specific for IE. We made the study of bacterial species associated with dental caries, a periodontal disease-causing bacteria, the investigation of infecting organism for opportunistic infections by using an examination for dental caries, an examination for periodontal disease, fence-sitting bacteria examination of BML company.