Elucidation of MHC polymorphism information in cynomolgus macaque(Macaca Fascicularis)

本研究采用桑格法和下一代测序法对食蟹猴（Macaca fascicularis，Mafa）Ⅰ类和Ⅱ类基因进行分型。利用该方法，我们发现了新的MHC等位基因，并估计了菲律宾人口的MHC单倍型的基因分型的200只动物。此外，我们从1222只动物的大规模筛选中鉴定了具有最常见MHC单倍型的5只纯合动物和43只杂合动物以及具有其他MHC单倍型的23只纯合动物。综上所述，本研究对食蟹猴MHC基因多态性进行了研究，为食蟹猴的医学研究奠定了基础。

We developed a genotyping methods on Macaca fascicularis(Mafa) class I and class II genes by Sanger and next generation sequencing. Using the methods we discovered new MHC alleles, and estimated MHC haplotypes in Filipino population from genotyping of the 200 animals. Moreover, we characterized five homozygous and 43 heterozygous animals that have the most frequent MHC haplotype and 23 homozygous animals that have the other MHC haplotypes from a large scale screening of 1222 animals. In summary, we characterized the Macaca fascicularis MHC polymorphisms and constructed research base for medical research in this research.