Development of non-invasive procedures for the diagnosis of chronic hepatitis and non-alcoholic steatohepatitis(NASH) using ultrasound technologies

评价非造影超声和超声造影（CEUS）对慢性肝炎和非酒精性脂肪性肝炎（NASH）的诊断性能。应用虚拟触摸组织定量技术（VTTQ）建立了一种诊断慢性肝炎的新方法。本文对超声造影诊断NASH进行了研究。在大鼠NASH模型上使用活体显微镜进行的观察显示，枯否细胞（KC）对Sonazoid的摄取并未随时间增加，这被认为是由于KC的吞噬能力降低所致。在疾病早期通过VTTQ诊断NASH提示了一些仍需探索的问题。

The diagnostic performance of non-contrast enhanced ultrasound and contrast enhanced ultrasound(CEUS) for chronic hepatitis and non-alcoholic steatohepatitis(NASH) were evaluated. A new diagnostic method for chronic hepatitis was developed using Virtual Touch Tissue Quantification(VTTQ). The diagnosis of NASH by CEUS using Sonazoid was studied. Observations using intravital microscopy on a rat NASH model revealed that the uptake of Sonazoid by Kupffer cells(KCs) did not increase over time, and that was considered due to the decreased phagocytic ability of KCs. The diagnosis of NASH by VTTQ at early stages of the disease suggested some issues that still need to be explored.