Chemical mutagenesis

为了建立家蚕随机诱变系统，研究了乙基亚硝基脲诱变家蚕的可能性。结果，ENU被证明是一种有用的化学来源。受精卵浸泡在ENU溶液中。对798例经ENU处理的个体在不同阶段的表型进行了评估。其中发现异常性状212例。为了调查突变体，我们从具有异常性状的个体兄弟姐妹杂交中选择了40对。在G2或G3代中，发现了20多个候选突变体。此外，我们还开发了一种利用CO2气体获得高倍率三倍体蚕种的方法。三倍体家蚕精子均为真核精子，可辅助真核精子受精。此外，我们还对具有snp信息和标记基因的突变体进行了连锁分析。我们还发现了野蚕（Bombyx mandarina）的新特征：黑蛹和大卵。

To develop random mutagenesis system in the silkworm, the possibility of mutagenesis by ethyl nitrosourea (ENU) was investigated. ENU was proved to be a useful chemical source as a result, . The fertilized eggs were immersed in ENU solution. The phenotypes of 798 individuals treated with ENU were evaluated at various stages. Abnormal characters were discovered in 212 individuals of them. To survey the mutant, we selected 40 pairs from sib-crossing of individuals having abnormal characters. In G2 or G3 generation, more than 20 mutants were discovered as mutant candidate. In addition, we developed one method to obtain triploid silkworm with high ratio by using CO2 gas. All of the sperm of triploid silkworm were apyrene sperm that can assist fertilization of eupyrene sperm. Moreover, we performed linkage analysis of mutants with SNPs information and marker gene. We also found new characteristics in wild silkworm, Bombyx mandarina: black pupa and big egg.