Proteomic analysis of global changes induced by deep hypothermia in both protein synthesis and phosphorylation.

低温治疗越来越多地用于保护大脑等器官免受缺血性损伤。虽然低温保护的主要机制可能是抑制代谢，但其多效性尚未完全了解。为了分析低温诱导的蛋白质组学变化，我们采用了基于荧光的二维（2d）差异凝胶电泳（DIGE）和基质辅助激光解吸/电离飞行时间（MALDI-TOF/TOF）串联质谱法。深低温使大鼠心肌中调节ATP合成或肌肉收缩的蛋白显著下调。低温还显著下调了大鼠肝脏内质网（ER）、线粒体、ATP结合、氨基酸代谢、尿素循环、氧化应激反应、抗凋亡、凋亡负调控等细胞成分和生物学过程或途径。这些变化与冬眠动物相似，除了内质网和细胞凋亡相关的途径，它们在冬眠哺乳动物中相反上调。这种差异需要在未来的研究中进一步研究。

Hypothermia is utilized with increasing frequency to protect organs such as the brain against ischemic injury. Though a principal mechanism of hypothermic protection would be suppression of metabolism, its pleiotropic effects are as yet incompletely understood. To analyze hypothermia-induced proteomic changes, we employed fluorescence-based two-dimensional(2-D) difference gel electrophoresis(DIGE) and matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF/TOF) tandem mass spectrometry. Deep hypothermia produced significant down-regulation of the proteins that regulates ATP synthesis or muscle contraction in rat heart muscle. Hypothermia also produced significant down-regulation of the following cellular components and biological processes or pathways in rat livers : endoplasmic reticulum(ER), mitochondria, ATP binding, amino acid metabolism, urea cycle, response to oxidative stress, anti-apoptosis, negative regulation of apoptosis. These changes were similar to those in hibernating animals except for both ER and apoptosis-related pathways, which are conversely up-regulated in hibernating mammals. This discrepancy needs to be investigated further in future research.