Examination of the behavior of the human leukemia cells in immunodeficient mice: For the safety improvement of the ovarian bank

在治疗前收集癌症患者卵巢组织的系统被称为“卵巢银行”。卵巢银行有望成为保障青少年癌症妇女生育能力的新技术。然而，在癌症治疗前使用卵巢组织的安全性检查尚未完成。我们进行了以下检查。1)利用免疫缺陷小鼠制备人白血病模型：我们将GFP基因导入人白血病细胞，并证实白血病细胞移植到模型小鼠。GFP诱导效率极低，且各细胞组间存在差异。引入GFP基因后，将白血病细胞移植到免疫缺陷小鼠体内。各细胞组移植后存活率不同。2)白血病细胞在模型小鼠中的行为：我们通过组织学检查和移植到其他免疫缺陷小鼠后的生存能力检查人白血病细胞在模型小鼠卵巢中的存在或不存在。不同细胞群的存活率差异很大。关于白血病细胞污染卵巢的可能性尚未得到明确的结论。3)辅助生殖技术模拟后小鼠白血病细胞污染的检测：我们通过模拟胚胎移植后的小鼠骨髓检测肿瘤细胞是否存在。

A system gathering the ovarian tissue from cancer patients before treatment is called as "ovarian bank". The ovarian bank is expected as a new technique which secure fertility of juvenile cancer women. However, the examination of the safety about using ovarian tissue before the treatment of cancer, is not yet done. We performed the following examination.1) Production of the human leukemia model by using immunodeficient mouse: We introduced GFP gene into human leukemia cells, and confirmed the transplant engraftment of the leukemia cells to a model mouse. The GFP induction efficiency is extremely low level and a difference was found every cellular group. Leukemia cells after introduction of GFP gene, were transplanted into immunodeficiennt mice. The survival rates after transplantation were different on every cellular group.2) Behavior of leukemia cells in the model mouse: We examined presence or absence of the human leukemia cells in the model mouse ovary, by histologic investigation and examination of the viability after transplantation into other immunodeficient mouse. The rate of survival is different greatly every cellular group. The clear conclusions were not obtained about the the probability of leukemia cells contamination to an ovary.3) The examination of the leukemia cells contamination in mouse after assisted reproductive technology simulation: We examined the presence or absence of tumor cells with the mouse bone marrow after transplantation of embryos following simulation.