Role of retinoic acid in the mechanism of skin wound healing

我们使用Cyp 26 b1基因敲除（KO）小鼠研究视黄酸（RA）在皮肤发育中的作用。在Cyp 26 b1-KO表皮中观察到皮肤屏障功能障碍。我们发现皮质层畸形，颗粒层缺乏透明角质颗粒。krtap 13，一个标志物的peridendum，也上调，并在后期阶段。虽然Cyp 26 b1-KO皮肤缺乏毛囊，但将皮肤移植到裸鼠可以诱导毛发形成。为了了解维甲酸对创面愈合的影响，我们进行了胎儿手术。在Cyp 26 b1-KO皮肤中，与正常皮肤相比，观察到愈合延迟。在这项研究中，我们发现RA对皮肤发育和伤口愈合至关重要。

We searched for a role of retinoic acid(RA) in the developmental skin using Cyp26b1 knockout(KO) mice. A skin barrier dysfunction was observed in the Cyp26b1-KO epidermis. We found malformation in the cornified layer and absence of keratohyaline granules in the granular layer. Krtap13, a marker of the periderm, was also up-regulated and last in later stages. Although the Cyp26b1-KO skin lacked hair follicles, skin grafting to a nude mouse could induce hair formation. We performed fetal operation to know the effect of RA on wound healing. In the Cyp26b1-KO skin, a delay of healing was seen compared with the normal one. In this study, we found RA is critical for skin development and wound healing.