Novel virus discovery from blood-feeding insects inhabiting Southeast Asian area

改进的病毒RNA序列快速测定系统（RDV）有助于各种类型病毒的鉴定和发现。在越南，从蚊子中分离出日本脑炎病毒、Dak Nong病毒（未分类的中脊病毒科）、库蚊黄病毒、南定病毒和班纳病毒。在菲律宾，从库蚊中分离出内盖夫病毒，并在蚊池中发现了几种传染性病原体。从日本库蠓中分离到沙蒙达病毒和沙氏病毒。根据这些病毒分离株的遗传信息，建立了基于PCR的病毒诊断方法。提示埃及伊蚊是菲律宾吕宋岛登革热的主要传播媒介。对拟除虫菊酯的敏感性较低。埃及人。阐明了拟除虫菊酯抗性基因在吕宋岛广泛传播。

Improved system for rapid determination of viral RNA sequences (RDV) contributed to the identification and discovery of various types of the viruses. In Vietnam, Japanese encephalitis virus, Dak Nong virus (unclassified Mesonidoviridae), Culex flavivirus, Nam Dinh virus and Banna virus were isolated from mosquitoes. In the Philippines, Negev virus was isolated from Culex vishnui, and several infectious agents were found in mosquito pools. Shamonda virus and Sathuperi virus were isolated from Culicoides biting midges in Japan. PCR-based virus diagnosis was established based on the genetic information of these virus isolates. It was suggested that Aedes aegypti is regarded as the primary vector of Dengue fever in Luzon island, Philippines. The sensitivity to pyrethroid insecticide was low in Ae. aegypti. It was clarified that the pyrethroid resistance gene is widely spread throughout Luzon island.