Real-time measurements of supramolecular structural change of biological macromolecules in photoreaction

圆偏振发光(CPL)代表样品的左、右圆偏振光的差示发射，反映了被激发荧光团的手性。该方法已被广泛应用于生物大分子的结构信息获取。为了将CPL光谱系统应用于DNA，我们将合成各种用于标记生物分子的发光探针。生物分子上的荧光探针的时间分辨CPL(TrCPL)测量使我们能够检测生物大分子在光反应中的超分子结构变化。在本研究期间，我们发表了6篇论文。

Circularly polarized luminescence (CPL) represents the differential emission of left and right circularly polarized light from a sample, being a reflection of the chirality of the excited fluorophore. This method has been used extensively to obtain structural information of biomolecules. In order to apply the CPL spectroscopic system to DNA, we will synthesize various luminescent probes for labeling biomolecules. Time-resolved CPL (TR-CPL) measurements of the fluorescence probes attached to biomolecules enable us to detect supramolecular structural changes of biological macromolecules in photoreaction. We have published 6 papers during this study duration.