Toward REVERSE PROTEOMICS : a selection platform of a peptide fragment that specifically inhibits the targeted protein-protein interaction.

本研究建立了一种筛选特异性抑制靶蛋白相互作用的多肽片段的有效方法。我已经证明了相互作用结构域，或负责结合表面形成的多肽，从任何一个靶向蛋白质可以是一个竞争性抑制剂对天然蛋白质之间的相互作用在体内。一种新的筛选系统与定量报告基因（工程真菌cDNA分泌β-半乳糖苷酶，LacA 3）也被开发，以促进定向进化的抑制剂片段。

An efficient methodology that facilitates selecting a polypeptide fragment that specifically inhibits the targeted protein-protein interaction has been established in this research. I have demonstrated an interaction domain, or a polypeptide responsible for the binding surface-formation, from either of the targeted proteins can be a competitive inhibitor against the interaction between the native proteins in vivo. A novel screening system with a quantitative reporter gene(an engineered fungal cDNA for secretory beta-galactosidase, LacA3) was also developed to facilitate a directed evolution of the inhibitor fragment.