Unraveling regulatory network in wood formation

利用只含拟南芥转录因子(Tf)基因的人工文库，建立了一种新型高效的酵母单/双杂交筛选系统。这一新系统使我能够非常有效地识别与特定DNA或蛋白质结合的转铁蛋白。我筛选了与木材形成相关的基因的调节区结合的TF，并确定了许多TF作为候选。这一结果逐渐使我描绘出转录调控网络的全貌。此外，我还在拟南芥中找到了一些拯救非木材突变体表型的因子，并对它们的生物学功能进行了分析。

New efficient improved yeast one-/two-hybrid screening system has been developed in which the artificial library containing only Arabidopsis genes for transcription factor(TF) is employed. This new system lets me identify TF binding to specific DNA or protein in very efficient manner. I screened TF binding to the regulatory region of genes involving to wood formation and identified many TFs as candidates. This result gradually makes me to draw entire picture of transcriptional regulatory network. Furthermore, I found some TFs rescuing phenotype of wood-less mutant in Arabidopsis and am analyzing their biological functions.