Isolation of pine wood nematode resistance gene in pines by comparative genomics

为了分离黑松对松材线虫的抗性基因，利用人工栽培作物的抗线虫基因(NR-基因)的序列信息，采用比较基因组学方法进行了研究。利用抗病品种、感病品种和人工交配群体对分离到的候选基因进行了表型和基因型的关联分析。结果表明，抗病品种中分离的等位基因与抗病有效相关。

To isolate resistance genes in Pinus thunbergii against the pine wood nematode (PWN), the comparative genomic approaches was conducted using the sequence information of nematode resistance gene (NR-gene) in cultivated crop. The isolated candidate gene was evaluated by an association analysis between genotype and phenotype using the resistant variety, the susceptible variety, and the artificial mating population. As a result, the isolated alleles in resistant variety showed an effective association with resistance against the PWN.