Three-dimensional structural and functional analysis of the Golgi apparatus by new method of scanning electron microscopy

将光学显微镜技术和扫描电子显微镜观察有效地结合起来，开发了一种新的高分辨率三维观察方法。这种方法特别适用于含有各种细胞的组织的研究，使迄今为止用扫描电子显微镜难以对垂体前叶细胞进行分类的方法成为可能。此外，用高分辨率扫描电子显微镜精确分析了高尔基体各前叶垂体细胞的三维超微结构及其与其功能的关系。

A novel high resolution three-dimensional observation method was developed by an effective combination of light microscopic technique and scanning electron microscopic observation. This method was especially useful for investigation of tissues containing various kinds of cell, enabling the classification of anterior pituitary cells which has hitherto been difficult by using scanning electron microscopy. Furthermore, the three-dimensional ultrastructure in relation to its function of each anterior pituitary cell of the Golgi apparatus was precisely analyzed by high-resolution scanning electron microscopy.