Functional characterization of two new sugar transporter families within the Major Facilitator Superfamily (MFS)

我们的研究小组几乎完全描述了拟南芥（AtSTPs）中一个由14个高度同源的成员组成的单糖转运蛋白家族。对于大多数atstp，我们可以证明一个特定的沉淀表达和定位在质膜上。在酵母细胞中的表达表明，大多数这些转运蛋白接受广泛的单糖底物。除了atstp外，在拟南芥基因组中还可以鉴定出6个不同的假定糖转运蛋白家族。其中一个家族与细菌木糖/H+同向转运体具有显著的同源性，而另一个家族与空泡转运体和哺乳动物促进体有关。我们希望通过研究这两个转运蛋白家族的转运特性、组织特异性和（亚）细胞定位以及个体转运蛋白的发育、空间和环境调控来描述这两个转运蛋白家族的功能特征。

Our group has almost completely characterized a family of monosaccharide transporters in Arabidopsis (AtSTPs) comprising 14 highly homologous members. For most AtSTPs, we could demonstrate a sink-specific expression and the localisation in the plasmamembrane. Expression in yeast cells showed that most of these transporters accept a broad range of monosaccharide substrates. In addition to the AtSTPs, 6 distinct families of putative sugar transporters can be identified within the Arabidopsis genome. One of these families shows significant homologies to bacterial xylose/H+ symporters, while another family is related to vacuolar transporters and mammalian facilitators. We wish to functionally characterize these two families by investigating the transport properties, the tissue-specific and (sub)cellular localizations as well as the developmental, spatial, and environmental regulation of the individual transporters.