Analysis of the HIF-1 activation upon infection with Bartonella henselae and other pathogens

亨塞巴尔通体是一种新兴的病原体，是细菌性血管瘤病和盆腔增生的病原体，这两种疾病都以内皮细胞增生为特征。B. henselae表现出对内皮细胞的趋向性，病原体可能侵入，持续存在和复制。通过蛋白质组学、反向遗传学和细胞实验，我们希望阐明(i)母鸡B.的致病性因子在与内皮细胞相互作用时表达，（ii）这些因子如何促进母鸡B.的侵袭和细胞内持久性，以及（iii）这些因子如何调节内皮宿主细胞。这些研究结果将为henselae感染的分子发病机制提供新的见解，并将证明兼性细胞内病原体如何调节内皮细胞以确保其栖息地。

Bartonella henselae, a new emerging pathogen, is the causative agent of bacillary angiomatosis and peliosis, both of which are characterized by the proliferation of endothelial cells. B. henselae exhibits a tropism for endothelial cells in which the pathogen may invade, persist and replicate. By means of proteomics, reverse genetics, and cellular experiments we want to elucidate (i) what pathogenicity factors of B. henselae are expressed upon interaction with endothelial cells, (ii) how these factors contribute to invasion and intracellular persistence of B. henselae, and (iii) how these factors modulate the endothelial host cells. The results of these investigations will provide new insights into the molecular pathogenesis of B. henselae infections, and will demonstrate how facultative intracellular pathogens can modulate endothelial cells in order to ensure their habitate.