Exploring the role of Reggie genes and microdomain signaling during zebrafish development

Reggie-1和Reggie-2是质膜相关蛋白和脂筏微结构域的特征性标志物，其似乎为神经元和许多其他细胞类型中的跨质膜信号传导提供组装功能平台。在大鼠和金鱼中，reggies在轴突生长和再生过程中上调。我们以前已经确定了四个reggie基因在斑马鱼，所有这些都是转录的早期发展，特别是在胚胎的眼睛，大脑和体节的形态发生。Hedgehog信号通路的组分是这些结构的图案化的主要参与者，并且还已知与脂筏相互作用。本文拟采用原位杂交、抗体染色和morpholino基因打靶相结合的方法研究斑马鱼reggie基因的功能。此外，我们将研究reggies和刺猬信号通路在轴突生长/再生过程中可能的功能关联，通过蛋白质共定位和共免疫沉淀培养细胞。

Reggie-1 and -2 are plasma membrane-associated proteins and characteristic markers of lipid-raft microdomains, which appear to provide assemble functional platforms for signaling across the plasma membrane in neurons and many other cell types. In rats and goldfish, reggies are upregulated during axon growth and regeneration. We have previously identified four reggie genes in the zebrafish, all of which are transcribed in early development, particularly during the morphogenesis of the embryonic eye, brain and somites. Components of the Hedgehog signaling pathway are major players in the patterning of these structures, and are also known to interact with lipid rafts. Here, we propose tho examine the function of zebrafish reggie genes by a combination of in-situ hybridizations, antibody stainings and morpholino gene targeting. In addition, we will examine the possible functional association between reggies and the Hedgehog signaling pathway during axon growth/regeneration, by means of protein co-localiszation and co-immunoprecipitation in cultured cells.