The effects of early exercise on brain plasticity after focal cerebral infarction in rats.

本研究的目的是探讨早期跑台训练对脑梗死大鼠的影响。本研究为康复医学提供了基础依据。本研究使用54 - 8周龄雄性Wistar大鼠，体重200- 300 g。通过动脉切开术将细丝（带钝头的4-0尼龙缝线）插入左颈总动脉。然后，它通过颈内动脉的内腔，卡在狭窄的近端大脑前动脉中，阻塞了大脑中动脉的起源。通过90分钟的细丝撤回建立再灌注。手术后第二天，将中风大鼠随机分为两组：运动组或非运动对照组。缺血后，大鼠在跑台上跑20 min，每天1次。所有大鼠术后均无运动协调和神经功能缺损。运动组运动行为和神经功能评分的改善优于非运动组。术后14 d运动组评分较非运动组明显提高（P<0.05）。运动员的中期因子（MK）的表达增加，与非运动员有或没有大脑中动脉闭塞3天。

The purpose of this study was to investigate the effects of early treadmill training after cerebral infarction in rats. This study might provide a basic evidence of rehabilitation medicine. Fifty-four- 8-week old male Wistar rats weighing 200-300g were used in this study. The filament (4-0 nylon suture with blunted tip) was inserted into the left common carotid artery by an arteriotomy. It was then passed up through the lumen of the internal carotid artery and lodged in the narrow proximal anterior cerebral artery, blocking the MCA at its origin. Reperfusion was established by withdrawal of the filament by 90 min. Next day after surgery, stroke rats were randomly assigned to two groups : treadmill-exercised group or non-exercised controls. After ischemia, the rats were received to run on a treadmill for 20 min once a day. All rats were observed the absence of motor coordination and neurological deficits after surgery. The improvement of the motor behavioral and the neurological score in the exercised groups was better than those in non-exercised groups. Particularly, the score in the exercised groups was significantly improved compared with those in non-exercised groups at 14 days after surgery (P<0.05). The runner had the increased expression of midkine (MK) over the non-runner with or without MCA occlusion for 3 days.