Stem cell culture system with immobilized signal proteins.

将细胞因子、生长因子等多种信号蛋白光固定于培养容器上，研究它们对干细胞的影响。固定化的LIF和MCP 1趋化因子使ES细胞处于未分化状态。活化素A高剂量固定化可促进ES细胞向内胚层细胞分化，低剂量固定化可促进ES细胞向中胚层细胞分化。固定的VEGF直接从小鼠iPS细胞生长CD31阳性细胞，而无需预先分选分化细胞。这些结果表明，干细胞的培养装置，可以调节分化的可能性。

Various signal proteins such as cytokines and growth factors were photo-immobilized on culture vessels to investigate the effect of them on stem cells. Immobilized LIF and MCP1 chemokine grow ES cells in undifferentiated state. Activin-A immobilized at high dose enhanced ES cells to differentiate into endodermal cells, and at lower dose to cells with mesodermal phenotype. Immobilized VEGF grow CD31 positive cells directly from mouse iPS cells without prior sorting of differentiating cells. These results suggest the possibility of culture devices for stem cells that can regulate differentiation.