Optical enzyme assay with a fluorogenic liposome

阳离子聚合物-两亲性阴离子络合物可以很容易地穿过脂质双层膜，利用荧光脂质体可以检测到这种荧光发射。竞争阴离子的存在阻碍了聚合物-反阴离子络合物的形成，应该会降低转位活性。化学反应中竞争阴离子的消耗或激活剂阴离子的产生导致了荧光反应。酶的活性，如蛋白激酶和乙酰胆碱酯酶，以及它们的抑制剂，很容易通过“肉眼”检测系统进行评估。此外，我们还成功地估计了酸奶中乳酸盐、牛奶中脂肪的风味浓度。这种简单的混合测量方法将是一种性价比高的多组分风味传感器。

Cationic polymer-amphiphilic anion complexes can easily cross the lipid bilayer membrane, which can be detected as fluorescence emission by using a fluorogenic liposome. Presence of competitive anions that hinder the formation of polymer-counteranion complexes should reduce the translocation activity. Consumption of the competitive anions or production of activator anions during chemical reactions led into fluorogenic response. Activities of enzymes, such as protein kinase and acetylcholine esterase, and their inhibitors were readily evaluated by the “naked eye” detection system. In addition, we successfully estimated the flavor concentrations of lactate in sour milk, lipid in cow’s milk. The simple mix-and-measure method will be a cost-effective multi-component flavor sensor.