Plant regeneration promoted by complete decapitation and controlled phenol metabolism in vitro

组织培养在植物科学和育种研究中是有效的，但在再生方面还没有建立起来，也不稳定。本文提出了一种新的组织培养方法，即在离体植物的子叶下切下胚轴。该方法将生长在缺氮培养基上的植株切下，移栽到含有抗褐变剂（AOPP）的培养基中，可促进植株的稳定再生。这些结果表明该方法可能适用于许多植物物种。

Tissue culture is effective in the study of plant science and breeding, but has not been established and unstable for regeneration. We developed a new tissue culture method in which hypocotyl is cut under cotyledons of a plant in vitro. When plant grown on a nitrogen deficient medium is cut by the method, and transplanted into a medium including a reagent to control browning (AOPP) promotes the regeneration with stability. These results show a possible adaptation of the method to many plant species.