Study of pathogenesis of chronic active EB virus infection and its treatment strategy.

EB病毒（EBV）基因型可以根据EBV核抗原2和3C以及BZLF 1启动子区（Zp）的基因序列差异来区分。在慢性活动性EBV感染（CAEBV）、传染性单核细胞增多症（IM）和EBV相关噬血细胞性淋巴组织细胞增多症（EBV-HLH）的日本患者中检查EBV亚型和BZLF 1 Zp变体。IM、CAEBV和EBV-HLH的EBV基因型均为1型。然而，BZLF 1 Zp的测序分析发现三个多态性Zp变体。这是第一个证据表明日本患者的EBV变异分布与其他亚洲患者相似。29 CAEBV相关的细胞基因先前通过来自CAEBV患者的体外培养细胞系的微阵列分析鉴定。在这项研究中，我们还调查了这些细胞基因的表达水平在三个EBV相关的疾病，包括CAEBV，IM和EBV-HLH，使用实时RT-PCR分析。

Epstein-Barr virus (EBV) genotypes can be distinguished based on gene sequence differences in EBV nuclear antigens 2 and 3C, and the BZLF1 promoter zone (Zp). EBV subtypes and BZLF1 Zp variants were examined in Japanese patients with chronic active EBV infection (CAEBV), infectious mononucleosis (IM), and EBV-associated hemophagocytic lymphohistiocytosis (EBV-HLH). EBV genotype in IM, CAEBV, and EBV-HLH all belonged to type 1. However, sequencing analysis of BZLF1 Zp found three polymorphic Zp variants. This is the first evidence that the EBV variant distribution in Japanese patients resembles that found in other Asian patients. Twenty-nine CAEBV-associated cellular genes were previously identified by microarray analysis of in vitro cultured cell lines derived from patients with CAEBV. In this study, we also investigated the expression levels of these cellular genes in the three EBV-related disorders including CAEBV, IM, and EBV-HLH, using real-time RT-PCR analysis.