Measuring human complement activation for antibody mediated rejection

在移植中，各种抗体引起抗体介导的排斥反应。人类白细胞抗原抗体的临床意义取决于其激活补体(C)的能力。目前尚不清楚激活人类C蛋白所需的人类白细胞抗原抗体。与标准的细胞毒作用相比，直接测定靶细胞上的C3b可以更好地表明人类白细胞抗原抗体激活人类C细胞的能力。人类C3b的沉积更多地受到特定血清/细胞组合的影响，而不是受免疫球蛋白的数量的影响，一些组合表现出高免疫球蛋白和低C3b，而另一些组合表现出低免疫球蛋白和高C3b。IVIG对补体有明显的剂量依赖性抑制作用。检测人类C组分可能会改善供受者相容的评估。静脉注射免疫球蛋白有助于减少抗体介导的排斥反应。

In transplantation, various antibodies cause antibody mediated rejection. The clinical significance of HLA antibodies depends on their ability to activate complement (C). The requirements of HLA antibodies to activate human C are not known. Direct C3b measurement on target cells may by a better indication of the ability of HLA antibodies to activate human C than standard cytotoxicity. Human C3b deposition was influenced more by the particular serum/cell combination under study than by the amount of IgG, with some combinations showing high IgG and low C3b and others showing low IgG and high C3b. IVIG produced significant dose-dependent complement inhibition. Measuring human C component may improve the assessment of donor-recipient compatibility. IVIG is useful for reducing antibody mediated rejection.