Development of novel strategy for fertility preservation using cryopreserved ovarian tissue

一种基于荧光激活细胞分选的方案被用于成人卵巢皮质组织，以纯化具有与原始生殖细胞一致的基因表达谱的罕见的有丝分裂活跃细胞。一旦在体外建立，这些细胞(卵原干细胞)可以扩增几个月，并可以自发产生卵母细胞，这是由形态、基因表达和单倍体(1n)状态决定的。将人OSCs注射到人卵巢皮质活检组织中，可以在异种移植到免疫缺陷雌性小鼠1-2周后形成包含卵母细胞的卵泡。因此，育龄妇女的卵巢与成年小鼠相似，拥有可以在体外繁殖的OSCs，也可以在体外和体内产生卵母细胞。

A fluorescence-activated cell sorting-based protocol was used with adult human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells. Once established in vitro, these cells (oogonial stem cells; OSCs) can be expanded for months and can spontaneously generate oocytes, as determined by morphology, gene expression and haploid (1n) status.Injection of the human OSCs into human ovarian cortical biopsies leads to formation of follicles containing oocytes 1-2 weeks after xenotransplantation into immunodeficient female mice. Thus, ovaries of reproductive-age women, similar to adult mice, possess OSCs that can be propagated in vitro as well as generate oocytes in vitro and in vivo.