The analysis of novel molecular targeted therapy inhibiting S100A4 for ovarian cancer

我们发现S100A4基因启动子到内含子区域-4 ~ +248处的缺氧反应元件（Hypoxia Response Element， HRE）在卵巢癌细胞中过表达是重要的。因此，低氧环境被认为上调了S100A4的表达。接下来，我们利用含有10000种化合物的化合物文库对抑制S100A4基因转录的化合物进行筛选。我们发现6种化合物显著抑制其基因的转录。这些化合物被认为是新型分子靶向治疗的候选化合物。

We revealed that the Hypoxia Response Element(HRE) existing in -4 to +248 on the region from promoter to intron in S100A4 gene was important for its over-expression in ovarian carcinoma cells. Thus, low oxygen environment was considered to up-regulate the expression of S100A4. Next, we performed the screening of compounds suppressing the transcription of S100A4 gene using compounds library containing 10,000 kinds of compound. We found 6 kinds of compound significantly suppressing the transcription of its gene. These were regarded as a candidate compound for novel molecular targeted therapy.