Comprehensive measurement of proteinases and proteinase inhibitors within osteoarthritic knee cartilage and synovial fluid collected from osteoarthritic knee joints

为了确定OA软骨中蛋白酶的量，从软骨基质中提取蛋白质，并通过ELISA和Luminex测定各种蛋白酶的浓度，以及内源性蛋白酶抑制剂的浓度。从OA膝关节收集滑液，并测定蛋白酶和内源性抑制剂的量。我们的分析结果表明，OA软骨含有各种蛋白酶，除了MMP-13和ADAMTS-4和5，这被认为是OA的主要蛋白酶。再次，我们的研究揭示了OA关节的滑液含有大量的MMP-1、2和3以及TIMP-1和2。

In order to determine the amounts of proteinases within OA cartilage, proteins were extracted from cartilage matrix, and concentrations of various proteinases were determined by ELISA and Luminex, together with those of endogenous proteinase inhibitors. Synovial fluid was collected from OA knees, and the amounts of proteinases and endogenous inhibitors were determined as well. The results of our analysis revealed that OA cartilage contains various kinds of proteinases besides MMP-13 and ADAMTS-4 and 5, which have been supposed to be primary proteinases in OA. Again, our study has revealed that the synovial fluid from OA joints contains considerable amounts of MMP-1, 2 and 3 together with TIMP-1 and 2.