Experimental research of the effect of nerve on negative pressure wound therapy and clinical application

本文研究了大鼠皮肤成纤维细胞和分化为交感神经节样神经元的PC12细胞在不同类型负压作用下的动力学变化。结果表明，间歇负压条件下真皮成纤维细胞的增殖和迁移能力比常压条件下更强，而连续负压条件下胶原凝胶的收缩能力比常压条件下更强。然后，其中真皮成纤维细胞和分化的PC12细胞共培养的胶原凝胶在间歇负压下比在大气压下收缩更多。结果提示，间歇负压能有效促进创面愈合。

Kinetic change of dermal fibroblasts of rats and differentiated PC12 cells like sympathetic ganglionic neuron was investigated under various types of negative pressure. The results indicated that proliferation and migration potencies of dermal fibroblasts were promoted under intermittent negative pressure more than under atmospheric pressure, and collagen gel including dermal fibroblasts contracted under continuous negative pressure more than under atmospheric pressure. Then, the collagen gel in which dermal fibroblasts and differentiated PC12 cells were co-cultured contracted under intermittent negative pressure more than under atmospheric pressure. The results suggested that intermittent negative pressure effectively promotes wound healing.