Investigation of biofilm-related molecules in Porphyromonas gingivalis

在本研究中，牙龈卟啉单胞菌血凝素HagB被发现是糖基化的，并参与这种微生物的生物膜形成。我们还发现从牙龈卟啉单胞菌培养上清中分离的外膜囊泡（OMV）不仅含有几种毒力因子如牙龈卟啉酶（包括Rgp和Kgp），而且还含有主要和次要的菌毛蛋白如FimA和MfaI。我们进一步证明了OMV引起口腔上皮细胞从细胞培养板上脱落，这是牙龈蛋白酶依赖性的。两者合计，我们建议可能的作用HagB和OMV在生物膜的形成和破坏牙周组织，分别在牙周疾病的发展。

In the present study, Porphyromonas gingivalis hemagglutinin HagB was found to be glycosylated and involved in biofilm formation of this microorganism. We also found that outer membrane vesicles (OMVs) isolated from the culture supernatant of P. gingivalis contained not only several virulence factors such as gingipains including Rgp and Kgp, but also the major and minor fimbrial proteins such as FimA and MfaI. We further demonstrated that the OMVs caused detachment of oral epithelial cells from the cell culture plate, which is gingipain-dependent. Taken together, we suggest a possible role of HagB and OMVs in biofilm formation and destruction of periodontal tissue, respectively, during development of periodontal diseases.