Analyses of segregation of chloroplast nucleoids

本研究通过LC-MS/MS分析鉴定了41个蛋白质为叶绿体类核相关蛋白。然后，对两个含量较高的未知功能蛋白cpNUC1和cpNUC3进行了分析。我发现：(1)cpNUC1蛋白在体外具有DNA紧凑能力，但不参与cpDNA的分离；(2)cpNUC3参与了rrn23 RNA的隐性断裂，但不参与cpDNA的分离。此外，我还揭示了叶绿体dna复制是由氧化还原而不是叶绿体分裂/细胞周期来调节的。

In this study, 41 proteins were identified as chloroplast nucleoid-associated proteins by LC-MS/MS analyses. And then, I analyzed about two high content unknown function proteins, cpNUC1 and cpNUC3. I found that (1) cpNUC1 protein has an ability of DNA compaction in vitro but is uninvolved in the cpDNA segregation, and that (2) cpNUC3 is involved in hidden break of rrn23 RNA but is uninvolved in the cpDNA segregation. In addition, I revealed that chloroplast DNA replication is regulated by the redox rather than chloroplast division/cell cycle