Molecular analysis for evaluation of genetic diversity and taxonomic relationships among populations of Japanese newts

我们调查了系统发育关系和估计的历史人口的日本火腹蝾螈，Cynops pyrrhogaster，从日本大陆使用1407 bp的线粒体DNA序列（NADH 6，tRNAglu，cyt B）和1208 bp的核DNA序列（Rag-1）基因。基于线粒体DNA的系统发育树揭示了该物种内的四个主要分支（北方，中部，西部和南部分支）。核基因除北方分支外，没有明显的地理结构模式，表明是不完全谱系分类的结果。我们开发了15个新的微卫星标记。pyrrhogaster使用下一代测序仪。使用这些标记在边界区域周围的遗传调查显示，CENTRAL分支的等位基因频率与西方分支的等位基因频率不同，表明这些分支在生殖上彼此隔离。

We investigated the phylogenetic relationships and estimated the historical demography of the Japanese fire-bellied newt, Cynops pyrrhogaster, from Japanese mainlands using 1407-bp sequences of the mitochondrial DNA (NADH6, tRNAglu, cyt b) and 1208-bp sequences of nuclear DNA (Rag-1) genes. Phylogenetic trees based on mitochondrial DNA revealed four major clades (NORTHERN, CENTRAL, WESTERN, and SOUTHERN clades) within this species. Nuclear gene presented no obvious patterns of geographic structure except for the NORTHERN clade, suggesting results of incomplete lineage sorting. We developed 15 novel microsatellite markers for C. pyrrhogaster using next generation sequencer. Genetic survey using these markers around a boundary region revealed allele frequencies of CENTRAL clade differ from those of WESTERN clade, suggesting these clades reproductively isolated from each other.