pproach for circadian oscillatory mechanisms based on phosphorylation rhythms of clock proteins

在哺乳动物的生物钟中，CLOCK和BMAL1与DNA结合并在转录反馈回路中发挥重要作用。在这项研究中，我们进行了ChIP-Seq分析，并在全基因组范围内确定了所有的clock结合区域。RNA-Seq分析显示了所有转录本的昼夜节律谱。此外，我们发现JNK是一种新的蛋白激酶，可以磷酸化CLOCK-BMAL1复合物，JNK3-KO小鼠的行为分析表明，JNK在生物钟的光调节中起关键作用。

In the mammalian circadian clockwork, CLOCK and BMAL1 bind to DNA and play an important role in the transcriptional feedback loops. In this study, we performed ChIP-Seq analysis and determined all the CLOCK-binding regions in genome-wide manner. RNA-Seq analysis showed circadian profiles of all the transcripts. Furthermore, we identified JNK as novel protein kinase for phosphorylation of CLOCK-BMAL1 complex, and behavioral analysis of JNK3-KO mice showed that JNK is a key player in the photic regulation of circadian clockwork.