Shed light on the flavonoid accumulation of fruit

为了鉴定与柿子中原花青素积累有关的DkDHD/SDH、DkSCPL1和DkSCPL2基因，我们利用农杆菌将这些基因转化到柿叶中，并利用大肠杆菌BL21（DE3）产生DkDHD/SDH进行互补实验。我们无法获得DkSCPLs的明确结果。对于DkDHD/SDH，通过反义DkDHD/SDH转化减少原花青素的产生，并且BL21 δ aroE::KanR （DE3）与pet3 - dkdhdsdh的互补表明该蛋白在莽草途径中起SDH的作用。其次，在变异前后对红葡萄品种施用ABA和IAA，以明确这两种植物激素对果实果皮花青素积累的影响。结果表明，ABA处理通过MybA转录因子诱导蓝紫色花青素的积累，而IAA处理则减少其积累。

In order to characterize DkDHD/SDH, DkSCPL1 and DkSCPL2, which is supposed to be involved in proanthocyanidin accumulation in persimmon fruit, we tried transformation of persimmon leaf with these genes using Agrobacterium tumefaciens, and also production of DkDHD/SDH with Escherichia coli BL21(DE3) for complementation assay. We could not obtain clear results for DkSCPLs. As for DkDHD/SDH, transformation by antisense DkDHD/SDH reduce the production of proanthocyanidin, and complementation of BL21 delta aroE::KanR (DE3) with pET3a-DkDHDSDH shows this protein works as SDH in shikimic pathway.Secondly, we applied ABA and IAA to red grape cultivars around varaison to clear the effect of these plant hormones at anthocyanin accumulation of berry skin. The results indicated that the ABA treatment induces the accumulation of blue-purple anthocyanins via MybA transcription factor, while the IAA treatment reduces it.