The analysis of recognition of pathogen infection and induction of hypersensitive reaction by plant receptor-type protein kinases

植物对病原菌衍生分子、激发子进行识别，并产生超敏反应（hypersensitive response， HR），作为对病原菌的一种保护性反应。植物富含半胱氨酸的受体样激酶与HR的诱导有关，本研究分析了其对病原菌的识别机制。在酵母均质液中发现了与CRK相互作用的候选启动子，利用x射线晶体学解析了CRK的三维结构，并用核磁共振波谱法确定了CRK的相互作用位点。

Plants recognize molecules derived from pathogen, elicitor, and cause hypersensitive response(HR) as one of protective reaction against pathogen. Plant cysteine-rich receptor-like kinases have been reported to relate the induction of HR, and its recognition mechanim of pathogen was analyzed in this study. A candidate for elicitor, interacted with CRK, was found in yeast homogenate, and 3-D structure of CRK was solved by X-ray crystallography and the interaction site of CRK was identified by nuclear magnetic resonance spectroscopy.