Establishment of multidrug therapy using Atovaquone against canine Babesiosis.

本研究旨在建立阿托瓦酮(ATV)对犬巴贝斯虫病病原体吉氏巴贝斯虫的有效使用方法。我们利用吉氏杆菌连续培养进行了一些实验：(1)线粒体功能分析，(2)分子分析，(3)其他药物的敏感性试验，(4)ATV与其他药物对吉氏杆菌的相互作用试验，以及(5)马拉酮(R)对犬急性吉氏杆菌感染的疗效评估。结果表明，该原虫对ATV的抗性可能与线粒体细胞色素b基因上的M121I有关，且抗ATV的吉氏芽胞杆菌对其他化学物质没有交叉抗性。ATV与其他药物联用对原虫增殖有协同作用。在这项研究中，还表明马拉酮(R)对狗的急性吉伯逊杆菌感染有很高的疗效。

The purpose of this study was to establish an effective usage of atovaquone(ATV) against Babesia gibsoni, which is a pathogen of canine Babesiosis. We performed some experiment using B. gibsoni continuous culture ;(1) analysis of mitochondrial function,(2) molecular analysis,(3) sensitivity test using other chemicals,(4) interaction test among ATV and other drugs against B. gibsoni, and(5) assessment of treatment efficacy of Malarone(R) against canine acute B. gibsoni infection. As the result, it was strongly suggested that the ATV resistance of this protozoa may related to M121I on mitochondorial cytochrome b gene, and also that ATV resistant B. gibsoni have not cross resistance against other chemicals. Combination of ATV and other drugs showed synergic to additive efficacy against the protozoal multiplication. In this study, it was also shown that Malarone(R) have high efficacy against acute B. gibsoni infection in dogs.