Rational molecular design and synthesis of near-infrared fluorescent probes for in vivo imaging of tumor hypoxia

为了可视化各种被认为与恶性肿瘤密切相关的肿瘤细胞的缺氧状态，我开发了一种在缺氧条件下发射近红外荧光的探针，适用于非侵入性生物成像。首先，为了利用低氧选择性还原代谢反应，在密度泛函理论的基础上，合理设计和合成了探针分子。然后，我用荧光显微镜下的培养细胞对它们进行了评估。因此，我发明了一种在低氧条件下特定地在肿瘤细胞中发出近红外荧光的分子。

To visualize the hypoxic status of various tumor cells which are considered to be closely related to malignancy, I developed a probe that emit near-infrared fluorescence, which is suitable for non-invasive bioimaging, under hypoxia. First, to utilize hypoxia selective reductive metabolic reaction, I designed and synthesized probe molecules rationally in the aid of calculation based on density functional theory. Then, I evaluated them using culture cells by fluorescent microscopy. As a result, I invented a molecule that emit near-infrared fluorescence in tumor cells under hypoxic condition specifically.