Development of specific and highly sensitive method to detect nitroxyl by mass spectrometry

建立一种特异、高灵敏度的硝酰基（HNO）的质谱检测方法。合成了一种含碱性基团的水溶性硫醇化合物，以增强MS信号强度。用多反应监测法（MRM）检测巯基化合物与HNO反应生成的亚磺酰胺。我们的研究结果表明，亚磺酰胺是一个有用的标志物检测HNO。

This study aimed to develop specific and highly sensitive method to detect nitroxyl(HNO) by mass spectrometry(MS). A water-soluble thiol compound containing basic moiety to enhance MS signal intensity was synthesized. Sulfinamide produced by the reaction of thiol compound with HNO was detected by multiple reaction monitoring(MRM). Our results suggested that sulfinamide is a useful marker for detection of HNO.