Detection of non-culturable Legionella species by amoebal co-culture from bath water samples.

在这项研究中，我们调查了非可培养军团菌的患病率在洗澡水样本使用变形虫共培养。采用阿米巴共培养法对71份水样进行军团菌检测。共培养法、PCR法和定性PCR法检出63份洗浴水样阳性，阳性率为88.7%，常规培养法检出12份水样阳性，阳性率为16.9%。我们的研究结果表明，洗澡水样品被军团菌，包括VBNC形式的高度污染。

In this study, we investigated the prevalence of non-culturable Legionella species in bath water samples using amoebal co-culture. Legionella species were detected by amoebal co-culture method from 71 water samples. A total of 63(88.7%) bath water samples were positive by amoebal co-culture with culture, PCR, and qualitative PCR methods, whereas 12(16.9%) water samples were positive by conventional culture method. Our results suggested that bath water samples were highly contaminated by Legionella bacteria including VBNC form.