stablishment of cell therapy in maxillary and mandibular bone tissue regeneration using canine induced pluripotent stem cells

我们报道了通过转录因子Oct 3/4、Sox 2、Klf 4和c-Myc将犬诱导多能干细胞转化为犬胚胎成纤维细胞（CEF）。在敲除血清替代和神经干细胞无血清培养基下，在MEF上培养犬iPS细胞。然后，对源自犬iPS细胞的神经样细胞进行流式细胞术，以分选p75和HNK 1。将分离的神经嵴（NC）细胞培养在含有成骨细胞分化因子的神经干细胞无血清培养基中。通过p75和HNK 1染色观察从神经样细胞中分离的NC细胞的分化。成骨诱导14天后，NC细胞ALP活性升高，可能向成骨样细胞分化。

We reported the generation of canine induced pluripotent stem cells by the transcription factors Oct3/4, Sox2, Klf4, and c-Myc into canine embryonic fibroblasts (CEFs). Canine iPS cells were cultured on MEFs under knockout serum replacement and neural stem cell serum free medium. Then, flow cytometry was performed on neural-like cells derived from canine iPS cells to sort for p75 and HNK1. Isolated neural crest (NC) cells were cultured in neural stem cell serum free medium containing osteoblast differentiation factor. NC cells isolated from neural-like cell were observed for differentiation based on p75 and HNK1 staining. ALP activity of NC cells was increased in the osteoblast-induction for 14 days, thus, NC cells might be differentiated to osteoblast-like cells.