A study on analysis of oral microbiota of oral cancer patients and application to management perisurgical period

本研究的目的是寻找口腔癌患者特有的口腔微生物区系，并利用患者的唾液研究口腔微生物区系是否随着癌症治疗的进行而发生变化。口腔微生物区系的数量在癌症治疗前最高，放化疗期间最低。虽然T-RFLP法分析放化疗中奈瑟氏菌和罗氏菌的比例有所增加，但用Ion PGM法对比例的增加没有进行统计学分析，提示放化疗和手术对口腔微生物区系的影响并不显著，不同患者口腔微生物区系的变化也不同。

The purpose of this study is to find oral microbiota peculiar to oral cancer patient and to investigate whether oral microbiota changes according to the cancer treatment by using the patient's saliva.An amount of oral microbiota was highest before starting cancer treatment, and lowest during chemoradiotherapy. Though the proportion of Neisseria species and Rothia species became higher under the chemoradiotherapy analyzed by using T-RFLP method, those increase of proportions were not significant statistically analyzed by using Ion PGM method.These findings suggest that the influence of chemoradiotherapy and surgery on microbiota was not significant, and changes of oral microbiota differ from patient to patient.