Application and exploring of the induction condition of secondary metabolites producing genes in Rhodococcus jostii RHA1

乔氏红球菌（Rhodococcus jostii） RHA1生长在多种芳香族化合物上，对多氯联苯（PCBs）等环境污染物具有很强的降解能力。基于对RHA1基因组的分析，与其他放线菌一样，在染色体和质粒上发现了包括聚酮合成酶（PKS）和非核糖体依赖肽合成酶（NRPS）在内的许多次级代谢产物基因。在本研究中，研究了这些基因的诱导条件，以了解这些基因的次生代谢物在特定条件下的作用。I型、III型、PKS-NRPS杂交种和质粒上的NRPSs主要在固体培养基上诱导。另一方面，II型PKSs在液体培养基上诱导。为了更好地了解放线菌的次生代谢，我们正在尝试分析这些次生代谢产物的化学成分。

Rhodococcus jostii RHA1 was grown on a broad range of aromatic compounds and vigorously degrades environmental pollutants, such as polychlorinated biphenyls (PCBs). Based on the genome analysis of RHA1, many secondary metabolites genes including polyketide synthase (PKS) and non-ribosomal dependent peptide synthase (NRPS) were found on chromosome and plasmids, as same as other actinomycetes. In this study, induction conditions of these genes were investigated to understand what isa role of secondary metabolites from these genes on particular conditions. Type I, type III, PKS-NRPS hybrid, and NRPSs on plasmid were mainly induced on solid medium. On the other hands, type II PKSs were induced on liquid medium. For understanding of the secondary metabolism in actinomycetes, we are now trying to analyze chemical composition of these secondary metabolites.