A study of 3D mechanical network of the spindle-like structure composed of microtubules and kinesins.

我们研究了由(1)有丝分裂运动蛋白和(2)或轴丝动力蛋白参与纤毛运动所驱动的运动。我们发现，kinesin-6和外臂dynein使滑动的微管绕其纵轴旋转。在来自任何来源的kinesin-6和外臂dynein中都没有观察到这些微管的螺旋运动。螺旋运动取决于缓冲液中的ATP浓度、Kinesin-6头部N-末端区域的磷酸化、结合蛋白或负载方向。我们的结果表明，微管电机的扭矩产生可能受到外部条件的调节。

We have examined that the motility driven by (1) kinesins functioning in mitosis and (2)or axonemal dyneins being involved in ciliary movement. We found that kinesin-6 and outer arm dynein rotated sliding microtubules around their longitudinal axis. These corkscrewing motion of microtubule has not been observed in kinesin-6 or outer arm dynein from any source. The corkscrewing motion depends on ATP concentration in the buffer,phosphorylation on N-terminal region of kinesin-6 head, binding protein or direction of load. Our results suggested that torque generation of microtubule-based motors may be regulated by external conditions.