Three-dimensional stress strain measurement by micro/nano mechanical test under second harmonic generation microscope

我们开发了一种二次谐波产生（SHG）显微镜技术，用于非接触低侵入观察胶原纤维的内应力，胶原纤维是细胞外基质的主要成分，为皮肤等软组织提供机械强度。我们证明了该技术的潜力，证明了胶原纤维的SHG光强度与SHG显微镜上的拉伸仪引起的内应力有关，SHG显微镜将超短脉冲激光聚焦在胶原纤维上。

We developed a second harmonic generation(SHG) microscope technic for non-contact and low-invasion observation of internal stress of collagen fibers, which is main components of extracellular matrix, and gives mechanical strength to soft tissue like skin. We demonstrated the potential of the technic by showing that the intensity of SHG light from collagen fibers had a dependence on the internal stress induced by tensile tester on SHG microscope, which focus ultra-short pulsed laser on collagen fibers.