Evaluation of the radiation effect on selective chromosome segregation in stem cells

我们研究了辐射对选择性染色体分离的影响，以及小鼠神经球细胞中显示选择性染色体分离的细胞数与p53表达之间的关系。结果表明，显示选择性染色体分离的细胞数量根据照射的X射线剂量而减少。这种减少可能与CD133阳性细胞数量的剂量依赖性减少有关。此外，在p53敲除（KO）小鼠神经球细胞中未观察到显示选择性染色体分离的细胞，而在p53敲除（KD）神经球细胞中这些细胞的数量显著减少，表明p53功能参与了选择性染色体分离的调节。

We studied an effect of radiation on selective chromosome segregation and the relationship between the number of cells showing selective chromosome segregation and p53 expression in mouse neurosphere cells. The result revealed that the number of cells showing selective chromosome segregation was reduced depending on irradiated X-ray doses. This reduction may be related to the dose-dependent decrease in number of CD133 positive cells. In addition, the cells showing selective chromosome segregation were not observed in p53 knock out (KO) mouse neurosphere cells and the number of those cells was significantly reduced in p53 knock down (KD) neurosphere cells, suggesting that the p53 function is involved in the regulation of selective chromosome segregation.