Design of nanostructured carbon film electrode for developing drug metabolizing screening chip

利用纳米碳材料研制了细胞色素P450修饰电极组成的电化学生物传感器，用于筛选药物及其抑制剂的代谢。首先，我们通过对溅射碳膜进行紫外光/臭氧处理，研制了纳米碳膜电极。该电极适用于胆红素氧化酶等小分子酶的直接电子转移，但对药物代谢酶细胞色素P450(CYP)的直接电子转移效果较差。然后，将碳纳米纤维(CNF)修饰电极和CYP修饰电极应用于药物代谢和抑制剂筛选。在-0.302V处观察到CYP和CNF之间的DET。电催化还原电流随药物的加入而增大。相反，在阻滞剂的存在下，电流大大降低。近年来，我们发现具有粗糙表面结构的多晶ITO电极适合作为药物代谢和抑制剂筛选传感器，甚至可以与CYP微球一起使用。

Electrochemical biosensors consisting of cytochrome P450 modified electrodes have been developed for screening the metabolism of a drug and its inhibitor by using nanostructured carbon materials. First, we developed nanostructured carbon film electrode by UV/ozone treatment of sputtered carbon film. This electrode is suitable for efficient direct electron transfer with small enzymes such as bilirubin oxidase but not good for cytochrome P450(CYP) which is well known drug metabolism enzyme. Then, carbon nanofiber(CNF) modified and CYP modified electrode was applied for drug metabolism and inhibitor screening. DET between CYP and CNF was observed at -0.302V. The electrocatalytic reduction current increased with the addition of drugs. In contrast, the current was greatly reduced in the presence of inhibitor. Recently, we found that polycrystalline ITO electrode with rough surface structure is suitable as drug metabolism and inhibitor screening sensor even with CYP microsome.