Development of completely autologous aortic valves by a novel concept of regenerative medicine

组织工程技术可能在未来心血管替代物的发展中发挥重要作用。体外组织工程组织的研究主要集中在支架材料、细胞培养及其条件上。作为一种可行的再生医学方法，本研究开发了无细胞装置，利用体内组织构建技术制备完全自体的体内组织工程心血管组织，无需任何人工支架。通过设计或光工程方法成功地增强了组织形成过程。采用材料工程方法获得生物相容性。我们使用3D打印机生产无细胞设备。体外功能和耐久性评估后，通过植入山羊体内，评估了该装置获得的瓣状组织的临床可行性。

Tissue-engineering techniques may play a prominent role in the future development of cardiovascular replacements. Research designed to create in vitro tissue-engineered tissues has focused on the scaffold materials, culturing of cells, and its condition. As a feasible, regenerative medicine approach, this study developed cell-free devices for the preparation of completely autologous in vivo tissue-engineered cardiovascular tissues without any artificial scaffolds by using of in-body tissue architecture technology. The tissue formation process was enhanced successfully by design or photo engineering approaches. The biocompatibility was obtained by material engineering approach. We used a 3D printer to produce cell-free devices. The clinical feasibility of the valve-shaped tissues obtained from the devices was evaluated by their implanting in goats after evaluating their function and durability in vitro.