Surface Desigh of Polymers by Using Photochemistry of Dithiocarbamate For the Control of Bioreaction

利用二硫代氨基甲酸盐光化学进行聚合物表面设计以控制生物反应

基本信息

批准号：
13450302
负责人：
NAKAYAMA Yasuhide
金额：
$ 7.87万
依托单位：
National Cardiovascular Center Research Institute
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

To investigate the characterizatics of interaction forces between proteins and end-grafted polymer surfaces, force-versus-distance curves (f-d curves) were measured between protein-fixed probe tips (albumin and lysozyme) and surfaces graft-polymerized with N,N-dimethylacrylamide or acrylic acid in an aqueous solution, using an atomic force microscope. The effects of grafted chain length, grafting density, and electrostatic properly of the grafted chain segments on the interaction forces in the processes of protein adsorption onto and desorption from the graft-polymerized surfaces were analyzed from the approaching and retracting traces of he observed f-d cures, respectively. The grafting state affects the adhesion characteristics of the protein and a polyanionic surface provides a significant adhesion force not only to a positively charged protein but also to a negatively charged one at physiological pH.On the other hand, vascular-like tubular tissues named biotubes consisting of autol … More ogous tissues were prepared using in vivo tissue engineering and their mechanical properties were evaluated for application as a small caliber artificial vascular prosthesis. The biotubes were prepared by embedding six kinds of polymeric rods (poly(ethylene)(PE), poly(fluoroacetate)(PFA), poly(methyl methacrylate)(PMMA), segmented poly(urethane)(PU), poly(vinyl chloride)(PVC) and silicone (Si)as a mold in six subcutaneous pouches in the dorsal skin of New Zealand White rabbits. For rods apart from PFA, biotubes were constructed after 1-month of implantation by encapsulation around the polymeric implants. The wall thickness of the biotubes ranged from about 50 to 200 μm depending on the implant material and were in the order PFA<PVC<PMMA<PU<PE. As for PE, PMMA, and PVC, the thickness increased after 3-month of implantation and ranged from 1.5 to 2 fold. None of the biotubes were ruptured when a hydrostatic pressure was gradually applied to their lumen up to 200 mmHg. The relationship between the intraluminal pressure and the external diameter, which was highly reproducible, showed a 'J'-shaped curve similar to the native artery. The tissue mostly consisted of collagen-rich extracellular matrices and fibroblasts. Generally, the tissue was relatively firm and inelastic for Si and soft for PMMA. For PMMA, PE and PVC the stiffness parameter of the biotubes obtained was similar to those of the human coronary, femoral and carotid arteries, respectively. Biotubes which possess the ability for wide adjustments in their matrices, mechanics, shape and luminal surface design can be applied for use as small caliber blood vessels and are an ideal implant since they avoid immunological rejection. Less

为了研究蛋白质与末端接枝聚合物表面之间相互作用力的特征，在蛋白质固定探针尖端（白蛋白和溶菌酶和溶菌酶）和用N，N-二氨基甲基丙烯酸或丙烯酸溶液中使用水平的酸度，在蛋白质固定探针尖（白蛋白和溶菌酶）之间测量了力相互距离曲线（F-D曲线）。在蛋白质吸附的过程中，移植链长度，移植密度和静电对移植链段的静电作用分别从接近和缩回的He观察到的F-D疗法中分析了蛋白质吸附和从移植物聚合表面解吸的影响。移植状态影响蛋白质的粘附特性和聚支持发表面，不仅为带正电的蛋白质提供了重要的粘合力，而且还可以在另一只手的物理pH.上，在物理上呈负电荷的蛋白质，还为蛋白质带负电荷的蛋白质提供了较小的粘液组织，这些粘液量是在物理ph.on中，由自动组织中的较小的机器人及其机械构成的机制及其机制构成，并具有较小的机器人的机制。假体。通过嵌入六种聚合棒（聚（乙烯）（PE），聚（氟乙酸）（PFA）（PFA），聚（甲基甲基丙烯酸甲酯）（PMMA），分段聚甲烷（PU）（PU）（PU）（PU），聚（乙烯基氯化物）（乙烯基氯）（PVC）（PVC）（pVC）和硅si si si si in si si in si si si si si，除了PFA外，白色的兔子是通过在植入聚合物周围的1个月后构建的。当静水压力逐渐施加到200 mmHg的腔内时，没有1.5到2倍的生物管。通常，组织相对牢固且无弹性，对于PMMA而言。对于PMMA，PE和PVC，所获得的生物管的刚度参数分别与人类冠状动脉，股骨和颈动脉的刚度相似。具有在其物品，力学，形状和腔表面设计中具有广泛调整能力的生物管可以用作小口径血管，并且是理想的植入物，因为它们避免了免疫学排斥。较少的