Surface Desigh of Polymers by Using Photochemistry of Dithiocarbamate For the Control of Bioreaction

利用二硫代氨基甲酸盐光化学进行聚合物表面设计以控制生物反应

• 批准号: 13450302
• 负责人: NAKAYAMA Yasuhide
• 金额: $ 7.87万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13450302/
• 关键词: Dithiocarbamate; Surface Polymerization; Photo Reaction; Photo Iniferter; Graft Polymerization; Living Polymerization; ジチオカルバメナ; 高分子表面

To investigate the characterizatics of interaction forces between proteins and end-grafted polymer surfaces, force-versus-distance curves (f-d curves) were measured between protein-fixed probe tips (albumin and lysozyme) and surfaces graft-polymerized with N,N-dimethylacrylamide or acrylic acid in an aqueous solution, using an atomic force microscope. The effects of grafted chain length, grafting density, and electrostatic properly of the grafted chain segments on the interaction forces in the processes of protein adsorption onto and desorption from the graft-polymerized surfaces were analyzed from the approaching and retracting traces of he observed f-d cures, respectively. The grafting state affects the adhesion characteristics of the protein and a polyanionic surface provides a significant adhesion force not only to a positively charged protein but also to a negatively charged one at physiological pH.On the other hand, vascular-like tubular tissues named biotubes consisting of autol … More ogous tissues were prepared using in vivo tissue engineering and their mechanical properties were evaluated for application as a small caliber artificial vascular prosthesis. The biotubes were prepared by embedding six kinds of polymeric rods (poly(ethylene)(PE), poly(fluoroacetate)(PFA), poly(methyl methacrylate)(PMMA), segmented poly(urethane)(PU), poly(vinyl chloride)(PVC) and silicone (Si)as a mold in six subcutaneous pouches in the dorsal skin of New Zealand White rabbits. For rods apart from PFA, biotubes were constructed after 1-month of implantation by encapsulation around the polymeric implants. The wall thickness of the biotubes ranged from about 50 to 200 μm depending on the implant material and were in the order PFA<PVC<PMMA<PU<PE. As for PE, PMMA, and PVC, the thickness increased after 3-month of implantation and ranged from 1.5 to 2 fold. None of the biotubes were ruptured when a hydrostatic pressure was gradually applied to their lumen up to 200 mmHg. The relationship between the intraluminal pressure and the external diameter, which was highly reproducible, showed a 'J'-shaped curve similar to the native artery. The tissue mostly consisted of collagen-rich extracellular matrices and fibroblasts. Generally, the tissue was relatively firm and inelastic for Si and soft for PMMA. For PMMA, PE and PVC the stiffness parameter of the biotubes obtained was similar to those of the human coronary, femoral and carotid arteries, respectively. Biotubes which possess the ability for wide adjustments in their matrices, mechanics, shape and luminal surface design can be applied for use as small caliber blood vessels and are an ideal implant since they avoid immunological rejection. Less

为了研究蛋白质与末端接枝聚合物表面之间的相互作用力特征，在水溶液中，用原子力显微镜测量了蛋白质固定探针（白蛋白和溶菌酶）与N，N-二甲基丙烯酰胺或丙烯酸接枝聚合物表面之间的力-距离曲线（f-d曲线）。通过观察到的f-d曲线的接近和回缩轨迹，分析了接枝链长、接枝密度和接枝链段的静电特性对蛋白质在接枝聚合物表面吸附和脱附过程中相互作用力的影响。接枝状态影响蛋白质的粘附特性，在生理pH下，聚阴离子表面不仅对带正电荷的蛋白质而且对带负电荷的蛋白质提供显著的粘附力。 ...更多信息 采用体内组织工程方法制备了猪组织，并对其作为小口径人工血管假体的应用进行了力学性能评价。将6种聚合物棒（聚乙烯（PE）、聚氟乙酸（PFA）、聚甲基丙烯酸甲酯（PMMA）、聚氨基甲酸酯（PU）、聚氯乙烯（PVC）和硅树脂（Si））作为模具包埋在新西兰白色家兔背部皮肤的6个皮下囊袋中制备生物管。对于除PFA之外的棒，在植入1个月后通过围绕聚合物植入物的包封来构建生物管。生物管的壁厚范围为约50至200 μm，取决于植入物材料，其顺序为PFA<PVC<PMMA<PU<PE。至于PE、PMMA和PVC，植入3个月后厚度增加，范围为1.5至2倍。当流体静压逐渐施加到其管腔直至200 mmHg时，没有生物管破裂。管腔内压力和外径之间的关系，这是高度可重复的，表现出类似于原生动脉的“J”形曲线。组织主要由富含胶原的细胞外基质和成纤维细胞组成。一般来说，硅的组织相对较硬且无弹性，而聚甲基丙烯酸甲酯的组织较软。对于PMMA、PE和PVC，获得的生物管的刚度参数分别与人冠状动脉、股动脉和颈动脉的刚度参数相似。生物管在基质、力学、形状和管腔表面设计方面具有广泛的调节能力，可用作小口径血管，并且由于它们避免了免疫排斥，因此是理想的植入物。少

项目成果

Shoji Ohya: "Thermoresponsive Artificial Extracellular Matrix for Tissue Engineering : Hyaluronic Acid Bioconjugated with Poly(N-isopropylacrylamide) Grafts"Biomacromolecules. 2. 856-863 (2001)

Shoji Ohya：“用于组织工程的热响应人工细胞外基质：透明质酸与聚（N-异丙基丙烯酰胺）移植物生物共轭”生物大分子。

Shoji Ohya: "Material design for an artificial extracellular matrix : cell entrapment in poly(N-isopropylacrylamide)(PNIPAM)-grafted gelatin hydwgel"J Artif Organs. 4. 308-314 (2001)

Shoji Ohya：“人工细胞外基质的材料设计：聚（N-异丙基丙烯酰胺）（PNIPAM）接枝明胶水凝胶中的细胞捕获”J Artif Organs。

W.G.Brodbeck, G.Voskerician, N.P.Ziats et al.: "In vitro leukocyte cytokine mRNA responses to biomaterials are dependent on surface chemistry"J Biomed Mater Res. 64A. 320-329 (2002)

W.G.Brodbeck、G.Voskerician、N.P.Ziats 等人：“体外白细胞细胞因子 mRNA 对生物材料的反应取决于表面化学”J Biomed Mater Res。

Yasuhide Nakayama, Takehisa Matsuda: "Photo-control of the interaction between endothelial cells and photo-cation generatable water-soluble polymers"Photochem Photobiol. 77. 480-486 (2003)

Yasuhide Nakayama、Takehisa Matsuda：“内皮细胞与光阳离子生成水溶性聚合物之间相互作用的光控制”Photochem Photobiol。

Yasuhide Nakayama, Takehisa Matsuda: "Photo-control of the interaction between endothelial cells and photo-cation generatable water-soluble polymers"Photochem Phologiol. 77. 480-486 (2003)

Yasuhide Nakayama、Takehisa Matsuda：“内皮细胞与光阳离子生成水溶性聚合物之间相互作用的光控制”Photochem Phologiol。