Identification of putative plant defense activators of novel mode of action.

植物防御激活剂是一种通过提高植物对病原菌侵染的免疫力来诱导抗病的化学物质。隐球蛋白是一种来源于病原卵菌的蛋白激发子，它在烟草BY-2细胞中诱导的防御信号通路作为一个简单的模型系统得到了广泛的研究。基于酶促产生活性氧物种(ROS)的时间模式与下游防御反应之间的相关性，我们建立了一种新的高通量筛选方法来鉴定新的植物防御激活剂的先导化合物。从筛选的11,000种化合物中，我们选择了58种候选激活剂。我们进一步研究了所选化合物对拟南芥植物防御相关基因表达的影响，并发现一些化合物可能以一种新的作用模式增强茉莉酸依赖的信号通路。

Plant defense activators are chemicals that induce disease resistance by enhancing plant immunity upon pathogen infection. Defense signaling pathways induced by cryptogein, a proteinaceous elicitor derived from a pathogenic oomycete, in tobacco BY-2 cells has been extensively studied as a simple model system. Based on the correlation between the temporal pattern of enzymatic production of reactive oxygen species (ROS) and downstream defense responses, we established a new high-throughput screening method to identify novel lead compounds for plant defense activators. From a total of 11,000 compounds screened, we selected 58 candidates for activators. We further examined the effects of selected compounds on the expression of defense-related genes in Arabidopsis plants, and discovered some chemicals that potentially enhance jasmonic acid-dependent signaling pathway as putative plant defense activators with a novel mode of action.