Molecular and functional profiling of vesicle trafficking pathways in Arabidopsis thaliana

植物的分泌途径控制着细胞膜腔室的稳态，并在细胞内腔室和细胞外腔之间运输大分子。尽管植物和其他真核生物之间的基本分泌机制是守恒的，但最近的研究表明植物在结构和功能上存在明显的差异。为了系统地研究植物分泌的关键因素，这些因素控制转运囊泡的形成，囊泡递送到靶膜，以及通过内部和外部线索在细胞内运输，我们提出了一种结合功能基因组学和蛋白质组学的方法。重点将放在拟南芥的Rab和ARF小G蛋白家族及其细胞伴侣上。这种方法将揭示植物分泌途径的组织蓝图的重要方面，其与其他真核生物的基本功能差异，并最终揭示其对植物形态和功能的贡献。

The secretory pathway of plants controls homeostasis of cellular membrane compartments, and transports macromolecules between intracellular compartments and the extracellular space. Despite conservation of the basic secretory machinery between plants and other eukaryotes, recent findings suggest distinct structural and functional differences in plants. To systematically study key players of plant secretion, which control formation of transport vesicles, vesicle delivery to target membranes, and intracellular trafficking by internal and external cues, we propose a combined functional genomics and proteomics approach. Focus will be on the Rab and ARF small G protein families and their cellular partners from Arabidopsis thaliana. Such an approach will uncover important aspects of the organisational blue print of the plant secretory pathway, its fundamental functional differences to other eukaryotes, and, finally, its contribution to form and function of plants.