Development of nano-sized DDS for nucleic acid drugs based on polypod-like structured nucleic acids

为了提高核酸药物的治疗效力，我们试图通过连接多足样结构核酸和树枝状纳米DDS来开发它们。我们发现，可以发育出3到8个豆荚的水龙，这些DNA纳米结构可以被免疫细胞有效地摄取，这取决于豆荚的数量。此外，我们成功地构建了DNA树状大分子，通过粘性末端连接多足类动物。随着这种DNA纳米结构的发展，免疫刺激的CpG DNA释放的细胞因子已经大大增加。因此，我们已经证明，这种方法对于提高核酸药物的效力是有用的。

To increase the therapeutic potency of nucleic acid drugs, we r tried to develop polypod-like structured nucleic acid, or polypodna, and dendritic nano-sized DDS by connecting them. We found that polypodna with 3 to 8 pods can be developed and these DNA nanostructures are efficiently taken up by immune cells depending on pod number. Furthermore, we succeeded in constructing DNA dendrimer by connecting polypodnas through their adhesive terminal ends. Cytokine release by immunostimulatory CpG DNA has been greatly increased by the development of such DNA nanostructures. Thus, we have shown that this approach can be useful for increasing the potency of nucleic acid drugs.