Biological functional analysis of novel axon guidance molecule LOTUS

在小鼠胚胎的外侧嗅束（LOT）发育过程中，LOTUS缺陷小鼠的轴突分支增加，而NgR 1缺陷小鼠的轴突分支减少。LOTUS基因敲除小鼠的轴突分支数量明显减少，提示Nogo-NgR 1相互作用可能诱导LOT轴突分支的形成; LOTUS基因敲除小鼠海马齿状回内侧区出现双皮质素阳性的锥体细胞迁移，提示LOTUS可能参与了发育中海马神经元的迁移

In developing lateral olfactory tract (LOT) in mouse embryos, axonal branch was increased in LOTUS-deficient mice, whereas the axonal branch was decreased in NgR1-deficient mice. Furthermore, the axonal branch was also decreased in their double knocking-out mice, suggesting that Nogo-NgR1 interaction may induce axonal branching of LOT.On the other hand, doublecortin-positive migrating pyramidal neurons were found in inside area of dentate gylus of hippocampus in LOTUS-deficient mice when compared with the wild type mice, suggesting that LOTUS may be involved in neuronal migration in developing hippocampus