The molecular biology and biochemistry of bacterial manganese oxidation

该项目将进一步加深对细菌锰(Mn2+)氧化的了解。将采用多学科的方法进一步研究恶臭假单胞菌Leptothrix sp.的Mn2+氧化系统的遗传和生化。和Pedommicrobium sp.这项工作将特别集中于比较来自无关细菌的Mn2+氧化系统。将结合分子生物学、蛋白质生物化学和光谱学进行研究。这将是第一次对细菌Mn2+氧化的酶进行如此详细的描述，并将导致对细菌锰氧化过程的更好理解。

This project will further the understanding of bacterial manganese (Mn2+) oxidation. A multi-disciplinary approach will be used to further investigate the genetics and biochemistry of the Mn2+-oxidising systems of Pseudomonas putida, Leptothrix sp. and Pedomicrobium sp. This work will focus in particular on comparing the Mn2+-oxidising systems from unrelated bacteria. A combination of molecular biology, protein biochemistry and spectroscopy will be used. This will be the first time that the enzymes of bacterial Mn2+-oxidation will have been characterised in such detail and will lead to a greater understanding of the process of bacterial manganese oxidation.